Human SMAD4/MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit
SKU: 9038710221

Human SMAD4/MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit

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Description

Human SMAD4/MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA KitThe Human SMAD4 MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit measures Smad4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Smad4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.

The Human SMAD4/MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit measures Smad4 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Smad4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Smad4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Smad4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 9038710221

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4.6 ★★★★★
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Tom is Tom
Draper, US
★★★★★ 4
Its a book!
Format: Paperback
Obviously, comparable to RTFM, marketed as more geared to responders, or Windows domain admins with a small amount of Linux in their network. My review assumes you know what the book you are shopping for is, you have read or heard about the RTFM and this. Awesome book, you'll likely find or be reminded of something very useful that you can use tomorrow based on flipping through it. Good - What you'd expect, condensed, long listing of commands with brief descriptions. Network, firewall, registry, services, etc etc etc. Command based AV scanning and lots more. Lots of stuff is based on "Basic" commands included in powershell or windows cmd, additional tools sysinternals suite, some typical third party network command tools, as well a few references to less common tools. Eh - Frighteningly short intros to some tools, but for brevity's sake seems necessary, I'd suggest readers try everything in a lab. Bad - Many commands are described from a very basic idea gearing up to fairly complex, complete, long commands, with lots of big font and double spacing. Little to zero instruction is provided for some stuff, for example, the reg query commands, which you can read and see what they do or are for. Lots of lines go to nearly the exact same command (like firewall rules), I feel like they'ed be better if they had more context. The font is bigger (than RTFM), I'd like it if the book made more use of tables, bolding and underlining, and italicizing do differentiate sections of text, along with a smaller font and no double-spacing. I feel like this just adds to the girth of the book. All in all, great book, I definitely recommend it for network or domain admins and defenders.
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Reviewed in the United States on March 13, 2017
A
Verified Purchase
Alechka
Bozeman, US
★★★★★ 5
It is in very good condition! Can't wait to get on and learn!
Format: Paperback
The Blue Team Field Manual came very fast in fact five days earlier then expected and it made me very happy! Not to mention that the book looks like it haven't even been used it was a little scratch and tiny dent on top cover but it is barely noticeable! Thank you so much! Will be coming back for some more books 📚
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Reviewed in the United States on June 1, 2023
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Daniel Miessler
Natrona Heights, US
★★★★★ 5
Crisp and Clean
Format: Paperback
This book does exactly what it promises. It's a super clean collection of tons of useful commands for any defend-focused team. It's not going to teach you any lessons, really, which is what I was looking for when I opened it. At first I was disappointed, but as I got deeper in I realized what the book was and what it wasn't, and it just got better and better. I wish there were more books of this kind. You don't have to write a dissertation in order to create something useful. Sometimes quick references are the best thing you can have.
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Reviewed in the United States on January 17, 2017
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John Farrell
West Palm Beach, US
★★★★★ 5
It is not a teaching tool but a good reference for someone who know but needs a fast ...
Format: Paperback
This is just what I was looking for. Just a fast lookup of commands needed to get the job done for Linux and Windows. It is not a teaching tool but a good reference for someone who know but needs a fast reminder. And it all corralated to just frame work. Love it
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Reviewed in the United States on April 12, 2017
C
Christopher Grehan
Dallas, US
★★★★★ 5
Packed with Information
Format: Kindle
Excellent companion into learning Godot and diving into the world of programming for games
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Reviewed in the United States on February 18, 2023

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