
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 5 - Jul 10
For Your Every Summer RSVP, with Code: SUMMER15
Description
Multi-Species 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA KitThe 5 HIAA (5 Hydroxyindoleacetic Acid) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Universal 5 HIAA. During the reaction, Universal 5 HIAA in the sample or standard competes with a fixed amount of Universal 5 HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 5 HIAA. Excess conjugate and unbound sample or standard are washed away,
The 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 5-HIAA. During the reaction, Universal 5-HIAA in the sample or standard competes with a fixed amount of Universal 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 5-HIAA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 5-HIAA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
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